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While MC4R KO mice generally demonstrate a more severe diabetic phenotype, male MC3R KO mice nevertheless do display mild impairments in insulin sensitivity and glucose control, along with increased adiposity (Butler et al., 2000

2.8 Protein carbonyl groups (PC) assay 1 mL of cytosol samples were mixed with a 200 L of 10 mM DNPH solution and were placed in the dark at room temperature (RT) for 60 min, with vortex-mixing every 15 min

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Zinc is vital for sperm production