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The primary pH-driven degradation concern for the copper peptide is copper dissociation below pH 5.0 due to histidine protonation

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Healthy control group: typically developing children without ASD

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Despite these limitations, WES continues to offer one of the most favorable costbenefit outcomes, offering almost the same percentage of diagnostic yield as Whole Genome Sequencing (WGS), but with and substantially lower bioinformatics workload (Nurchis et al., 2023)