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In addition, protein contents of the liver homogenate and cellular fractions were measured by the method of Lowry et al

Several techniques have been developed for the evaluation of the BBB such as the in vivo brain uptake index (BUI) method (88), in situ brain perfusion (89), cerebral microdialysis technique (90, 91), and cell uptake studies in the in vitro models of the BBB (92)

A great rule of thumb is to separate your actives

Garza Trevio et al

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