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Following incubation in serum-free medium containing 20 M DCFH-DA for 20 min at 37C, cells in each well were washed three times with PBS, digested by pancreatic enzymes at 37C for 3 min and immediately subjected to ROS measurement by flow cytometry analysis using Cytomics FC 500 MCL (Beckman Coulter, Inc., Brea, CA, USA)

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Chace, Carnitine treatment inhibits increases in cerebral carnitine esters and glutamate detected by mass spectrometry after hypoxia-ischemia in newborn rats., Stroke , vol

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