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Thakur D, Singh A, Prabhakar PK, Meghwal M, Upadhyay A

it was shown 30 years ago by Trojan and colleagues that this defect could be rescued in rat high grade glioma (HGG) cells by IGF-1 depletion, rendering the cells non-tumorigenic and able to induce CD8 + cytotoxic T-cell infiltration and regression of unmodified tumors in syngeneic animals 13

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Even if solution appears clear and colorless throughout the 30-day period, microbiological growth or chemical breakdown may be occurring invisibly