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For inputs, a fraction of this worm suspension was briefly spun down and Buffer A was replaced with 3 volumes of lysis buffer (25 mM HEPES-KOH pH 7.5, 150 mM KCl, 0.5% NP-40, 0.1% Triton X-100, protease and phosphatase inhibitors) and crushed with a metal douncer on ice with 100 strokes
Smoking and cardiac structure and function in the elderly: the ARIC study (Atherosclerosis Risk in Communities)
"Our sexuality, our gender, our identity were never quite understood
Emphasis was placed on the health consequences of smoking, such as impairment of physical performance, cardiovascular disease, pulmonary disease, and nicotine addiction
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