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rather they exhibit significant cross-talk ( Complement activation by smoking: possible suspects Concerning the potential tobacco chemicals that might activate the complement system, while the data available thus far are limited, activation of the classical pathway by tobacco glycoprotein (TGP)- which is derived from tobacco leaves- and a similar substance from cigarette smoke condensate- referred to as TGP-S- was explored ( Smoldering tobacco products produce particulate matter (PM), which is a major indoor air pollutant that has been linked to multiple diseases, including cancer ( C 3 - / - ) mice ( ex vivo and in vivo through the rise in both C3a and C5a, which was found to be mainly mediated by fine particulate matter PM 2.5 ( Tobacco smoking was also linked to a profile of toxic metals such as arsenic (As), cadmium (Cd), chromium (Cr), nickel (Ni), and lead (Pb) ( As was highlighted earlier in this review, tobacco smoking is linked/contains a host of different chemicals, some of which are known to be highly toxic (114, 115), which makes it possible to investigate the impact of some of these chemicals on the complement system, using curated databases such as the comparative toxicogenomic database (CTD) to delineate possible impacted pathways (116)

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Gudakhu usage was limited to female participants, preventing gender-based comparisons for this particular product