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WT samples for each peptide-spectra match by dividing each TMT reporter ion intensity by the average intensity for the channels corresponding to WT control (3 per TMT sample)

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Subsequently, the chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm

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Ther Deliv 5:709733 Illum L (2004) Is nose-to-brain transport of drugs in man a reality