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Traditionally, food supplements were often blamed for contamination, but as methods of analysis become increasingly sophisticated and sensitive, there is a greater likelihood that contamination can be traced to other sources

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Real-time qPCR was performed using an Applied Biosystems QuantStudio 12 K Flex real time PCR system (Thermo Fisher Scientific, San Jose, CA, USA) with THUNDERBIRD SYBR qPCR Mix (Toyobo), 6 pmol of primers, and aliquots of cDNA

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