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After 1 h, virus was aspirated, and cells were overlayed with carboxy-methyl cellulose (CMC) containing media 0.6% CMC, MEM supplemented with 1 Penicillin/Streptomycin (Gibco), 2% FBS, 1 mM sodium pyruvate (Gibco), 1 NEAA (Gibco), 0.3% sodium bicarbonate (Gibco), and 1 GlutaMAX (Gibco)