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IF microscopy (ALI wholemount IF labeling) ALI cultures on transwell inserts were rinsed with 1 PBS, fixed for 15 min in 3.2% PFA (no sucrose) at room temperature or 10 min in methanol at 20 C, rinsed twice more with 1 PBS and stored at 4 C

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Recording your recollections in writing can be useful evidence if you cannot recall the exact details at a later date