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Lentiviral transduction by VectorBuilder (Chicago, USA), SigmaAldrich (MO, USA) and SCBT (Santa Cruz Biotechnology, CA, USA) was performed as previously described [19] The transfection efficiency of the shRNAs was evaluated by Western blotting

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[16] In May 2005, Sheetz began offering their new MasterCard PayPass with RFID technology, [17] [18] [19] and was one of the first retailers to accept such technology, ahead of McDonald's, Arby's, CVS, and rival 7-Eleven, all of which introduced it nationally in 2014

[94] That same year, she divorced Minnelli, [95] and on June 8, 1952, she married her tour manager and producer Sidney Luft in Hollister, California