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Although ED is often underreported in African countries due to cultural stigma, lack of awareness, and limited data, its estimated rate of occurrence in Sub-Saharan Africa and different African countries are shown in Table 1 below: Table 1: showing prevalence of erectile dysfunction in some African countries and in the sub-Saharan Africa region

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For each replicate: (1) for determination of nicotine, 0.1 mL of the MTBE phase was placed in a ~ 2 mL brown glass autosampler vial with a septum cap then diluted using 0.9 mL MTBE