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[10] Empty spittoons or cups regularly

Nicotine content of electronic cigarettes, its release in vapour and its consistency across batches: regulatory implications

The evidence suggests an association and plausible biological mechanisms, but more research is needed to fully characterise this relationship

At a molecular level, smoking affects key signaling pathways such as nuclear factor-kappa B (NF-kB) and mitogen-activated protein kinases (MAPK) and induces epigenetic modifications like DNA methylation and histone modification [22]

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