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For changes in nicotine intake, we will calculate the mean change in salivary cotinine concentrations from baseline to 2-month follow-up for each group and the difference and 95% CI will be computed using linear regression with adjustment for GP practice

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For example, cigarette smoking is a major risk factor for many fatal diseases such as coronary heart diseases, cerebral vascular accident, lung cancer, and chronic obstructive pulmonary disease (WHO, 2011)