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Maintaining analytical stability and repeatability, quality control (QC) samples were meticulously prepared by pooling 10 L from each sample, systematically inserted for analysis every 5 samples

qRT-PCR and immunoblot analyses confirmed robust overexpression of Chac1 in liver (Supplemental Figure 1B and Figure 1C)

Most patients notice reduced appetite within the first week

Average trial results cannot predict an individual's gastrointestinal response

Possible reasons this topic attracts attention include changes in body weight, nutrition, stress, energy balance, hormonal signalling, medication changes and underlying health conditions