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For TEM preparation, samples were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4), followed by 1% osmium tetroxide post-fixation

(Note: While oral Epsom salt is an approved laxative, we recommend it strictly as a bath soak for GLP-1 users due to its harsh effects on an already sensitive stomach)

Journal of Biological Chemistry 269 , 2375723763 (1994)

Previous research has shown that children with ASD exhibit lower levels of reduced glutathione and abnormalities in transsulfuration metabolites, which are crucial for detoxification, antioxidant defense, and immune function