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Thein, [email protected] This article was submitted to Red Blood Cell Physiology, a section of the journal Frontiers in Physiology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

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The safest reconstitution method involves: (1) Using sterile technique (clean workspace, alcohol swabs, sterile syringes), (2) Using bacteriostatic water for injectionnever tap water, distilled water, or saline, (3) Allowing peptide vials to equilibrate to room temperature before reconstituting, (4) Injecting bacteriostatic water slowly down the vial wall rather than directly onto the powder, (5) Avoiding shaking or vigorous agitation, and (6) Storing reconstituted peptides refrigerated (2-8C) and protected from light

It is essential to recognize these limitations when interpreting the findings of our study