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Immunoprecipitation and immunoblotting Cells were collected in lysis buffer (0.25% Triton X-100 in PBS and 1:1000 protease inhibitor mixture) and sonicated for 12 s, followed by centrifugation at 16,100 g for 10 min

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In a heap of artists who take their craft very seriously, heres a guy with a penchant for public nudity, shameless drunkenness, and slovenly classic-rock covers peppered with the shouted words SUCK MY DICK! So while some find his behavior repugnant, others are enthralled with his youthful abandon

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So, this bill will raise the tax on cigarettes from $1 to $3 per pack - a 300% rate increase, according to Axios