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Similar treatment, however, resulted in skin papilloma development in hairless Swiss mice and the extract promoted skin papilloma formation in DMBA initiated Swiss mice.[] Prolonged application of an aqueous extract of chewing tobacco and lime or gutka to initiated or uninitiated Swiss Bare mouse skin failed to induce skin tumors, while topical application of gutka extract in tumor promotion and progressor stage increased the yield of papillomas and the rate of conversion of papilloma to carcinoma.[] Topical application of bidi tobacco extract to the back skin of Swiss Bare mice for 40 weeks or only once as a tumor initiator failed to induce skin papillomas in mice receiving TPA as a tumor promoter