Ratiometric image analysis was conducted using a Python script (available from The background was subtracted for each channel and the ROIs (=transfected cells) were determined by thresholding or with the Cellpose 40 algorithm in the channel of the FP
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Results revealed differential abundance at various taxonomic levels, including 1 at the phylum level ( Patescibacteria ), 1 at the class level ( Saccharimonadia ), 4 at the order level ( Staphylococcales , Saccharimonadales , Corynebacteriales and Bifidobacteriales ), 6 at the family level ( Staphylococcaceae , Saccharimonadaceae , Rikenellaceae , Marinifilaceae , Corynebacteriaceae and Bifidobacteriaceae ), 11 at the genus level ( unclassified_f__Anaerovoracaceae , UCG-005 , Staphylococcus , Odoribacter , norank_f__Christensenellaceae , Intestinimonas, Corynebacterium , CHKCI002 , Candidatus_Saccharimonas , Bifidobacterium and Alistipes ), and 17 at the species level (Only Bacteroides barnesiae has complete annotation information)
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Avoid Heavy Exercise: Avoid intense physical activities that might cause excessive sweating for the first 24 hours
