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Although prolonged treatment with oral N-acetylcysteine (NAC) prevents acute exacerbations of chronic bronchitis, it remains controversial for the treatment of COPD (91, 103105)
This increased permeability allows lipopolysaccharides (LPS), food antigens, and microbial byproducts (PAMPs) to translocate into circulation, triggering systemic immune activation and placing further burden on mast cells 18
6, pp.522-528 [online] Available at: Bozack, A.K., Howe, C.G., Hall, M.N., Liu, X., Slavkovich, V., Llievski, V., Lomax-Luu, A.M., Parvez, F., Siddique, A.B., Shahriar, H., Uddin, M.N., Islam, T., Graziano, J.H
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The existing methods for phenotypic selection of genetically modified mammalian cells are mainly based on three types of phenotypic markers: an exogenous drug resistance gene that permits screening for positive cells, a fluorescent protein, such as GFP, that allows isolating positive cells by FACS (fluorescence-activated cell sorting) (Chen et al., 1999), or a membrane protein that enables antibody-based immunomagnetic selection of positive cells (Plouffe et al., 2015)
