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Crumb WJ Jr, Clarkson CW (1990): Characterization of cocaine-induced block of cardiac sodium channels

Sample collection Pups were anesthetized with isoflurane and decapitated on their fifth day of life

Address methylation bottlenecks Distinguish low SAM from high SAH Review homocysteine, adenosine-related metabolism and kidney function Support transsulfuration and glutathione when appropriate Avoid automatic folate or methyl-donor treatment 6

An allergic reaction is possible if you are sensitive to sulfites

However, standard practice recommends using reconstituted peptide solutions within 7-14 days when stored at 2-8C, or maintaining frozen aliquots for longer-term storage