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The reaction mixture contained 1 ml of sample and 2 ml of TBA-TCA-HCl reagent (0.37% thiobarbituric acid, 0.25 N hydrochloric acid and 15% trichloroacetic acid), placed in boiling water bath for 15 min, cooled at room temperature and centrifuged for 10 min

Exploring novel strategies to improve anti-tumour efficiency: The potential for targeting reactive oxygen species

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Hepatology 71, 15751591

The PEN format refers to a laboratory applicator designed for precise and reproducible reagent dispensing in controlled test procedures