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The study, which was published in the JCO Oncology Practice journal, looked at University of Pennsylvania Health System records from more than 111,000 women ages 45 to 80 over the last several years who were overweight or obese, to see who went on to develop breast cancer

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The tissues were homogenized in ice-cold buffer, centrifuged for 30 min at 10,000 g, and the supernatant was kept at -80C for neurotransmitter (ACh, AchE, GABA, 5-HT, and dopamine), oxidative stress markers (MDA), and antioxidant status (GSH, CAT, and T-SOD) determinations