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The region of interest used for analysis was always a well-defined cytosol compartment excluding vacuolar regions

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For example, the compound 13 (racemic) is a very week IRAP inhibitor (Lukaszuk et al., 2011) and compound 14 with a 4-hydroxydiphenylmethane scaffold as a substitute for Tyr 2 is essentially inactive (Figure 4) (Andersson et al., 2008)

doi: 10.1016/j.diabres.2011.10.029 5 CosentinoFGrantPJAboyansVBaileyCJCerielloADelgadoVet al