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The duration of this trial was 30 weeks, and participants were given metformin as a background treatment

Here is a general timeline of what to expect: After 1-2 sessions: Skin may feel smoother and more hydrated

The concentrations of protein extracts were determined by using the BCA Protein Assay Kit (Thermo Fisher Cat #23225), then protein samples were mixed with 3 loading buffer and loaded in equal amounts into the wells of 412% Bis-Tris protein gels (Bio-Rad) to transfer the proteins to PVDF membranes (Millipore)

doi: 10.1046/j.1365-2982.2002.00311.x

What gets missed in the protein conversation at large is that many of our favorite protein sources are also fatty