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With standard L-glutathione a significant amount simply does not survive digestion

Darkness in the Human Gene and Protein Function Space: Widely Modest or Absent Illumination by the Life Science Literature and the Trend for Fewer Protein Function Discoveries Since 2000

Varying amounts of monobromobimane stock solution (0-300 l) were added to respective cuvettes already containing 5 l of the enzyme solution (protein concentration of 810 g ml -1 ), glutathione (100 l of stock solution) and phosphate buffer (3.0 ml)