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Human islets were maintained in culture for up to 48 h in RPMI medium containing 10% (vol./vol.) FCS, 1% (vol./vol.) penicillin/streptomycin and 5 mmol/l glucose

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Behaviorally, BALB/c mice display elevated anxiety and reduced social interaction compared to other strains, traits that mirror emotional (Mehla et al., 2022) and behavioral changes often observed in Alzheimers patients, such as anxiety, impaired social interaction, and disinhibition (Mitrea et al., 2022)

By blocking DPP-4 enzymatic activity, linagliptin increases the half-life of the incretin hormones, which in turn stimulates increased secretion of insulin by pancreatic -cells and reduces secretion of glucagon by pancreatic -cells