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Finally, smoking tobacco leaves a stale, tell-tale scent that is hard to disguise

Its believed that Rodrigo de Jerez, one of the crew of the Santa Maria, was the first European to actually smoke tobacco and he helped introduce it to the Old World

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Gel images evaluation Autoradiography films were scanned by means of densitometry and analyzed with Image/Gel Plotting analysis software (National Institutes of Health, Bethesda, Md) to determine band intensities