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In addition, the cell lines and primary cells are mostly cultured in non-physiological 2D conditions

The related damage can result in implantation failure, recurrent pregnancy loss, preterm labor and/or placenta accreta [78]

With respect to NHR-49 , codon-optimized constructs for full-length NHR-49 and a fragment spanning residues 1155 were cloned into the pET-28a(+) vector (GenScript, Piscataway, NJ)

Epithelial and endothelial damage, in turn, causes translocation of pro-inflammatory mediators and bacterial products, worsening systemic inflammation [23,43]